A highly versatile and easily configurable system for plant electrophysiology

被引:6
作者
Gunse, Benet [1 ]
Poschenrieder, Charlotte [1 ]
Rankl, Simone [2 ]
Schroeeder, Peter [2 ]
Rodrigo-Moreno, Ana [3 ]
Barcelo, Juan [1 ]
机构
[1] Univ Autonoma Barcelona, Fac Biociencias, Lab Fisiol Vegetal, Carrer Vall Moronta S-N, E-08193 Bellaterra, Spain
[2] Deutsch Forschungszentrum Gesundheit & Umwelt Gmb, Helmholtz Zentrum Munchen, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany
[3] Polo Scientifico, Dept Plant Soil & Environm Sci, Viale Idee 30, I-50019 Florence, Italy
来源
METHODSX | 2016年 / 3卷
关键词
Plant root; Membrane potential; Ion flux measurement; Electrophysiology;
D O I
10.1016/j.mex.2016.05.007
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In this study we present a highly versatile and easily configurable system for measuring plant electrophysiological parameters and ionic flow rates, connected to a computer-controlled highly accurate positioning device. The modular software used allows easy customizable configurations for the measurement of electrophysiological parameters. Both the operational tests and the experiments already performed have been fully successful and rendered a low noise and highly stable signal. Assembly, programming and configuration examples are discussed. The system is a powerful technique that not only gives precise measuring of plant electrophysiological status, but also allows easy development of ad hoc configurations that are not constrained to plant studies. We developed a highly modular system for electrophysiology measurements that can be used either in organs or cells and performs either steady or dynamic intra-and extracellular measurements that takes advantage of the easiness of visual object-oriented programming. High precision accuracy in data acquisition under electrical noisy environments that allows it to run even in a laboratory close to electrical equipment that produce electrical noise. The system makes an improvement of the currently used systems for monitoring and controlling high precision measurements and micromanipulation systems providing an open and customizable environment for multiple experimental needs. (C) 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
引用
收藏
页码:436 / 451
页数:16
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