PLANT-REGENERATION VIA SOMATIC EMBRYOGENESIS IN GINGER

被引：44

作者：

KACKAR, A ^{[1
]}

BHAT, SR ^{[1
]}

CHANDEL, KPS ^{[1
]}

MALIK, SK ^{[1
]}

机构：

[1] NATL FACIL PLANT TISSUE CULTURE,NBPGR CAMPUS,NEW DELHI 110012,INDIA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1993年 / 32卷 / 03期

关键词：

CALLUS CULTURE; HISTOLOGY; YOUNG LEAF; ZINGIBER-OFFICINALE;

D O I：

10.1007/BF00042291

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Embryogenic callus cultures of ginger were induced from young leaf segments taken from in vitro shoot cultures. Among the four auxins tested in Murashige & Skoog medium, dicamba at 2.7 muM was most effective in inducing and maintaining embryogenic cultures. Efficient plant regeneration was achieved when embryogenic cultures were transferred to Murashige & Skoog medium containing 8.9 muM benzyladenine. Histological studies revealed various stages of somatic embryogenesis characteristic of the monocot system. The in vitro-raised plants have been established in soil.

引用

页码：289 / 292

页数：4

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