ROD OPSIN CDNA SEQUENCE FROM THE SAND GOBY (POMATOSCHISTUS-MINUTUS) COMPARED WITH THOSE OF OTHER VERTEBRATES

被引:38
作者
ARCHER, SN
LYTHGOE, JN
HALL, L
机构
[1] UNIV BRISTOL, DEPT BIOCHEM, BRISTOL BS8 1TH, AVON, ENGLAND
[2] UNIV BRISTOL, DEPT ZOOL, BRISTOL BS8 1TH, AVON, ENGLAND
关键词
D O I
10.1098/rspb.1992.0037
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The absorbance spectra of rods from the sand goby were measured by using microspectrophotometry. Analysis of the averaged spectra shows that the rod visual pigment has a maximum absorbance (lambda(max)) at approximately 501 nm. A sand goby retinal cDNA library was constructed and then screened with a partial sand goby rod opsin clone obtained by the polymerase chain reaction (PCR). The screening of the library yielded a full length rod opsin clone. The cDNA sequence and deduced amino acid sequence of this clone are compared with those of other vertebrate rod opsins.
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页码:19 / 25
页数:7
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