COMBINATION OF SUPERCRITICAL FLUID CHROMATOGRAPHY WITH THIN-LAYER CHROMATOGRAPHY ON A SEMIPREPARATIVE SCALE

被引:13
作者
WUNSCHE, L
KELLER, U
FLAMENT, I
机构
[1] Research Laboratories, Firmenich SA, CH-1211 Geneva
来源
JOURNAL OF CHROMATOGRAPHY | 1991年 / 552卷 / 1-2期
关键词
D O I
10.1016/S0021-9673(01)95970-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Semi-preparative supercritical fluid chromatography (SFC) is a very valuable tool for the separation of unknown complex mixtures, as it may allow the isolation of sufficient amounts of individual constituents for H-1 and C-13 NMR spectroscopic identification. Semi-preparative SFC uses large-diameter packed columns and requires an efficient collection system. The performances of 4.6, 10 and 21 mm I.D. columns were compared and three different collection methods were tested for their efficiency: collection in pressurized stainless-steel tubes or in specially shaped glass vials, and condensation of the effluent at the starting line of a semi-preparative thin-layer chromatographic plate. The good yields that characterize the first two techniques could not be achieved with the third. The plate could, however, be developed, leading to a two-dimensional semi-preparative chromatographic separation. The spots must be localized on the plate in a non-destructive way in order to recover the samples. A general method is proposed, using berberine chloride, and also a more specific method, which allows the selective detection of certaion classes of compounds.
引用
收藏
页码:539 / 549
页数:11
相关论文
共 26 条
  • [1] Alkio M, 1988, 1ST P INT S SUP FLUI, P389
  • [2] PREPARATIVE SUPERCRITICAL FLUID CHROMATOGRAPHY
    BERGER, C
    PERRUT, M
    [J]. JOURNAL OF CHROMATOGRAPHY, 1990, 505 (01): : 37 - 43
  • [3] SUPERCRITICAL FLUID FRACTIONATION OF PETROLEUM-DERIVED AND COAL-DERIVED MIXTURES
    CAMPBELL, RM
    LEE, ML
    [J]. ANALYTICAL CHEMISTRY, 1986, 58 (11) : 2247 - 2251
  • [4] BIOSYNTHETIC-STUDIES OF MARINE LIPIDS .25. BIOSYNTHESIS OF DELTA-9(11)-STEROLS AND DELTA-7-STEROLS AND SAPONINS IN SEA-CUCUMBERS
    CORDEIRO, ML
    DJERASSI, C
    [J]. JOURNAL OF ORGANIC CHEMISTRY, 1990, 55 (09) : 2806 - 2813
  • [5] LC GC, SFC GC, AND SFE GC - INTERFACING
    DAVIES, IL
    RAYNOR, MW
    KITHINJI, JP
    BARTLE, KD
    WILLIAMS, PT
    ANDREWS, GE
    [J]. ANALYTICAL CHEMISTRY, 1988, 60 (11) : A683 - &
  • [6] FLAMENT I, 1988, 1ST P INT S SUP FLUI, P465
  • [7] FRIED B, 1986, CHROMATOGRAPHIC SCI, V35
  • [8] MICROCOLUMN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY THIN-LAYER CHROMATOGRAPHY FOURIER-TRANSFORM INFRARED SPECTROMETRY
    FUJIMOTO, C
    MORITA, T
    JINNO, K
    [J]. JOURNAL OF CHROMATOGRAPHY, 1988, 438 (02): : 329 - 337
  • [9] MICRO-HPLC/TLC/FTIR
    FUJIMOTO, C
    MORITA, T
    JINNO, K
    SHAFER, KH
    [J]. JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS, 1988, 11 (11): : 810 - 814
  • [10] PERFORMANCE, DATA AND RESULTS WITH VARIOUS CHROMATOGRAPHIC SYSTEMS AND VARIOUS DETECTION PATTERNS IN HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY
    HALPAAP, H
    RIPPHAHN, J
    [J]. CHROMATOGRAPHIA, 1977, 10 (11) : 643 - 650