LARGE-SCALE PURIFICATION OF MEMBRANE-CONTAINING BACTERIOPHAGE PRD1 AND ITS SUBVIRAL PARTICLES

被引：52

作者：

BAMFORD, JKH ^{[1
]}

BAMFORD, DH ^{[1
]}

机构：

[1] UNIV HELSINKI, DEPT GENET, ARKADIANKATU 7, SF-00100 HELSINKI 10, FINLAND

来源：

VIROLOGY | 1991年 / 181卷 / 01期

基金：

芬兰科学院;

关键词：

D O I：

10.1016/0042-6822(91)90501-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

PRD1 is a dsDNA virus that infects Escherichia coli and Salmonella typhimurium. The genome is a linear molecule with 5′ covalently linked terminal protein. The virus has a lipid membrane inside the protein coat. We describe the large-scale purification of the virus using a zonal rotor and the yields and quality of the virus and its subviral assemblies for subsequent biophysical measurements. © 1991.

引用

页码：348 / 352

页数：5

共 24 条

[1] STRUCTURE OF THE LIPID-CONTAINING BACTERIOPHAGE-PRD1 - DISRUPTION OF WILD-TYPE AND NONSENSE MUTANT PHAGE PARTICLES WITH GUANIDINE-HYDROCHLORIDE [J].

BAMFORD, D ;

MINDICH, L .

JOURNAL OF VIROLOGY, 1982, 44 (03) :1031-1038

[2] STUDIES OF VIRUS STRUCTURE BY LASER RAMAN-SPECTROSCOPY .33. STRUCTURAL STUDY OF THE LIPID-CONTAINING BACTERIOPHAGE PRD1 AND ITS CAPSID AND DNA COMPONENTS BY LASER RAMAN-SPECTROSCOPY [J].

BAMFORD, DH ;

BAMFORD, JKH ;

TOWSE, SA ;

THOMAS, GJ .

BIOCHEMISTRY, 1990, 29 (25) :5982-5987

[3] COMPARISON OF THE LIPID-CONTAINING BACTERIOPHAGE-PRD1, BACTERIOPHAGE-PR3, BACTERIOPHAGE-PR4, BACTERIOPHAGE-PR5 AND BACTERIOPHAGE-L17 [J].

BAMFORD, DH ;

ROUHIAINEN, L ;

TAKKINEN, K ;

SODERLUND, H .

JOURNAL OF GENERAL VIROLOGY, 1981, 57 (DEC) :365-373

[4] COLLAGENOUS PROTEINS MULTIPLY [J].

BAMFORD, DH ;

BAMFORD, JKH .

NATURE, 1990, 344 (6266) :497-497

[5] CHARACTERIZATION OF THE DNA-PROTEIN COMPLEX AT THE TERMINI OF THE BACTERIOPHAGE PRD1 GENOME [J].

BAMFORD, DH ;

MINDICH, L .

JOURNAL OF VIROLOGY, 1984, 50 (02) :309-315

[6] CAPSOMER PROTEINS OF BACTERIOPHAGE PRD1, A BACTERIAL-VIRUS WITH A MEMBRANE [J].

BAMFORD, JKH ;

BAMFORD, DH .

VIROLOGY, 1990, 177 (02) :445-451

[7]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[8] THE VIRION OF THE LIPID-CONTAINING BACTERIOPHAGE PR4 [J].

DAVIS, TN ;

MULLER, ED ;

CRONAN, JE .

VIROLOGY, 1982, 120 (02) :287-306

[9]

DAVIS TN, 1983, VIROLOGY, V126, P600

[10] LIPID-CONTAINING BACTERIOPHAGE-PR4 - STRUCTURE AND LIFE-CYCLE [J].

LUNDSTROM, KH ;

BAMFORD, DH ;

PALVA, ET ;

LOUNATMAA, K .

JOURNAL OF GENERAL VIROLOGY, 1979, 43 (JUN) :583-592

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