(+)-TUBOCURARINE IS A POTENT INHIBITOR OF CATION CHANNELS IN THE VACUOLAR MEMBRANE OF CHENOPODIUM-RUBRUM L

被引：17
作者：
WEISER, T
BENTRUP, FW
机构：
[1] Botanisches Institut I der Justus-Liebig-Universität, D-6300 Giessen
来源：
FEBS LETTERS | 1990年 / 277卷 / 1-2期
关键词：
PATCH-CLAMP TECHNIQUE; PLANT VACUOLE; POTASSIUM CHANNEL; TONOPLAST; (+)-TUBOCURARINE; CHENOPODIUM-RUBRUM L;
D O I：
10.1016/0014-5793(90)80849-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of the acetylcholine antagonist and channel blocker (+)-tubocurarine on the calcium-dependent slow vacuolar (SV) cation channels in the tonoplast of suspension-cultured cells of Chenopodium rubrum L. was examined using the patch-clamp technique. In whole-vacuolar recordings the drug strongly suppressed the potassium conductance (EC50:6-mu-M) and altered the kinetics of channel inactivation. In excised membrane patches (+)-tubocurarine evokes channel-'flickering' without affecting the single-channel conductance (approx. 80 pS).
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页码：220 / 222
页数：3
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