ADENYL CYCLASE IN CELL-FREE EXTRACTS OF ESCHERICHIA COLI

被引:15
作者
BRANA, H
机构
[1] Department of Bacterial Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences
关键词
D O I
10.1007/BF02872776
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The adenyl cyclase enzyme system was detected in the cells of Escherichia coli disrupted by sonic treatment. This enzyme activity is located mainly in the cell fraction sedimenting at 2,000×g, i.e. in the cytoplasmic membrane fraction. A prolonged sonication treatment of the cell suspension was followed by the disappearance of activity in the membrane preparation. The pH optimum of the adenyl cyclase in Escherichia coli was on the alkaline side, around pH 9. © 1969 Academia, nakladatelství Československé akademie věd.
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页码:185 / +
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