CHARACTERIZATION OF NITRIC-OXIDE SYNTHASES IN NONADRENERGIC NONCHOLINERGIC NERVE CONTAINING TISSUE FROM THE RAT ANOCOCCYGEUS MUSCLE

被引：103

作者：

MITCHELL, JA ^{[1
]}

SHENG, H ^{[1
]}

FORSTERMANN, U ^{[1
]}

MURAD, F ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,SCH MED,DEPT PHARMACOL,CHICAGO,IL 60611

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 1991年 / 104卷 / 02期

关键词：

NITRIC OXIDE; ARGININE; CYCLIC GMP; NG-NITRO-L-ARGININE; ENDOTHELIUM-DERIVED RELAXING FACTOR; GUANYLYL CYCLASE; 5,6,7,8-TETRAHYDROBIOPTERIN;

D O I：

10.1111/j.1476-5381.1991.tb12422.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Tissue homogenates prepared from rat anococcygeus muscle converted L-arginine to L-citrulline indicating the presence of nitric oxide (NO) synthase. NO synthase activity was also found in crude and partially-purified soluble and particulate fractions prepared from the homogenates. Both soluble and particulate NO synthase were dependent on NADPH, 5,6,7,8-tetrahydrobiopterin and calcium, and inhibited by N(G)-nitro-L-arginine. Tissue homogenates or crude cytosolic and membrane fractions from rat vas deferens, which does not contain NO releasing non-adrenergic non-cholinergic neurones, had no NO synthase activity.

引用

页码：289 / 291

页数：3

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