HIGH-SENSITIVITY OF GLUTAMATE UPTAKE TO EXTRACELLULAR FREE ARACHIDONIC-ACID LEVELS IN RAT CORTICAL SYNAPTOSOMES AND ASTROCYTES

被引:179
作者
VOLTERRA, A [1 ]
TROTTI, D [1 ]
CASSUTTI, P [1 ]
TROMBA, C [1 ]
SALVAGGIO, A [1 ]
MELCANGI, RC [1 ]
RACAGNI, G [1 ]
机构
[1] UNIV MILAN, DEPT ENDOCRINOL, I-20133 MILAN, ITALY
关键词
GLUTAMATE UPTAKE; ASTROCYTES; SYNAPTOSOMES; ARACHIDONIC ACID; SYNAPTIC MODULATION; EXCITOTOXICITY;
D O I
10.1111/j.1471-4159.1992.tb09411.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
By using both synaptosomes and cultured astrocytes from rat cerebral cortex, we have investigated the inhibitory action of arachidonic acid on the high-affinity glutamate uptake systems, focusing on the possible physiological significance of this mechanism. Application of arachidonic acid (1-100-mu-M) to either preparation leads to fast (within 30 s) and largely reversible reduction in the uptake rate. When either melittin (0.2-1-mu-g/ml), a phospholipase A2 activator, or thimerosal (50-200-mu-M), which inhibits fatty acid reacylation in phospholipids, is applied to astrocytes, both an enhancement in extracellular free arachidonate and a reduction in glutamate uptake are seen. The two effects display similar dose dependency and time course. In particular, 10% uptake inhibition correlates with 30% elevation in free arachidonate. whereas inhibition greater-than-or-equal-to 60% is paralleled by threefold stimulation of arachidonate release. In the presence of albumin (1-10 mg/ml), a free fatty acid-binding protein, inhibition by either melittin, thimerosal, or arachidonic acid is prevented and an enhancement of glutamate uptake above the control levels is observed. Our data show that neuronal and glial glutamate transport systems are highly sensitive to changes in extracellular free arachidonate levels and suggest that uptake inhibition may be a relevant mechanism in the action of arachidonic acid at glutamatergic synapses.
引用
收藏
页码:600 / 606
页数:7
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