A RAPID, SEMIAUTOMATED SDS-PAGE IDENTIFICATION SYSTEM FOR ORAL ANAEROBIC-BACTERIA

被引：15

作者：

SLAYNE, MA ^{[1
]}

ALDRED, MJ ^{[1
]}

WADE, WG ^{[1
]}

机构：

[1] SCH DENT CARDIFF,DEPT ORAL SURG MED & PATHOL,HEATH PK,CARDIFF CF4 4XY,WALES

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1990年 / 68卷 / 04期

关键词：

D O I：

10.1111/j.1365-2672.1990.tb02889.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

SDS‐polyacrylamide gel electrophoresis is a useful technique in bacterial differentiation and identification. A rapid, semi‐automated SDS‐PAGE system (Phast System) was assessed for identification of formate‐fumarate‐requiring, asaccharolytic, Gram‐negative oral anaerobes. The system permitted loading, separation and staining of gels within 2 h. Percentage similarities between strains were determined using correlation coefficients and cluster analysis. The protein profiles were sufficiently reproducible provided that distorted profiles were disregarded. Strains were successfully separated into their species, with the exception of Bacteroides ureolyticus NCTC 10939, which appeared to be distinct from other strains of that species. Twenty‐nine unidentified formate‐fumarate‐requiring, sub‐gingival plaque strains were suitably clustered with the standard strains as verified by a series of physiological and biochemical tests. and accepted 25 July 1989 Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：391 / 395

页数：5

共 10 条

[1] INCREASED SENSITIVITY FOR COOMASSIE STAINING OF SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS USING PHASTSYSTEM DEVELOPMENT UNIT [J].

HEUKESHOVEN, J ;

DERNICK, R .

ELECTROPHORESIS, 1988, 9 (01) :60-61

[2] CLASSIFICATION OF CORYNEBACTERIUM SPECIES FROM AXILLARY SKIN BY NUMERICAL-ANALYSIS OF ELECTROPHORETIC PROTEIN-PATTERNS [J].

JACKMAN, PJH .

JOURNAL OF MEDICAL MICROBIOLOGY, 1982, 15 (04) :485-492

[3]

JACKMAN PJH, 1987, METHOD MICROBIOL, V19, P209

[4] IDENTIFICATION AND GROUPING OF BACTERIA BY NUMERICAL-ANALYSIS OF THEIR ELECTROPHORETIC PROTEIN PATTERNS [J].

KERSTERS, K ;

DELEY, J .

JOURNAL OF GENERAL MICROBIOLOGY, 1975, 87 (APR) :333-342

[5] POLYACRYLAMIDE SLAB GEL-ELECTROPHORESIS OF SOLUBLE-PROTEINS FOR STUDIES OF BACTERIAL FLORAS [J].

MOORE, WEC ;

HASH, DE ;

HOLDEMAN, LV ;

CATO, EP .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1980, 39 (04) :900-907

[6] CHARACTERIZATION OF WOLINELLA SPP, CAMPYLOBACTER-CONCISUS, BACTEROIDES-GRACILIS, AND EIKENELLA-CORRODENS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

TANNER, ACR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1986, 24 (04) :562-565

[7] WOLINELLA GEN-NOV, WOLINELLA-SUCCINOGENES (VIBRIO-SUCCINOGENES-WOLIN ET-AL) COMB-NOV, AND DESCRIPTION OF BACTEROIDES-GRACILIS SP-NOV, WOLINELLA-RECTA SP-NOV, CAMPYLOBACTER-CONCISUS SP-NOV, AND EIKENELLA-CORRODENS FROM HUMANS WITH PERIODONTAL-DISEASE [J].

TANNER, ACR ;

BADGER, S ;

LAI, CH ;

LISTGARTEN, MA ;

VISCONTI, RA ;

SOCRANSKY, SS .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1981, 31 (04) :432-445

[8]

TANNER ACR, 1986, INT J SYSTEMATIC BAC, V34, P275

[9] NUMERICAL-ANALYSIS OF ELECTROPHORETIC PROTEIN-PATTERNS OF BACTEROIDES-UREOLYTICUS CLINICAL ISOLATES [J].

TAYLOR, AJ ;

COSTAS, M ;

OWEN, RJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (04) :660-666

[10] THE IDENTIFICATION OF BACTEROIDES-UREOLYTICUS FROM PATIENTS WITH NONGONOCOCCAL URETHRITIS BY CONVENTIONAL BIOCHEMICAL TESTS AND BY DNA AND PROTEIN ANALYSES [J].

TAYLOR, AJ ;

DAWSON, CA ;

OWEN, RJ .

JOURNAL OF MEDICAL MICROBIOLOGY, 1986, 21 (02) :109-116

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