NMR-STUDIES OF THE INTERACTION OF BLEOMYCIN WITH (DC-DG)3

被引：13
|
作者：
GAMCSIK, MP [1 ]
GLICKSON, JD [1 ]
ZON, G [1 ]
机构：
[1] APPL BIOSYST INC,FOSTER CITY,CA 94494
来源：
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | 1990年 / 7卷 / 05期
关键词：
D O I：
10.1080/07391102.1990.10508550
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The interaction of bleomycin A2 and Zn(II)-bleomycin A2 with the oligonucleotide (dC-dG)3 has been monitored by nuclear magnetic resonance spectroscopy. Binding of the drug to the oligonucleotide is indicated by an upheld shift of the bithiazole proton resonances consistent with partial intercalation of this group between base pairs. The effect of temperature and ionic strength on the binding of both free bleomycin and the Zn(II) complex has been studied. Consistent with earlier studies on polynucleotides, the rate of exchange between the free drug and the drug-oligonucleotide complex is rapid on the1H NMR chemical shift time scale. Binding of the oligonucleotide induced changes in resonances assigned to protons in the metal-binding region of Zn(II)-bleomycin. Intermolecular nuclear Overhauser effect enhancements between bleomycin and the oligonucleotide have not been detected. © Taylor & Francis Group, LLC.
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页码：1117 / 1133
页数：17
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