EFFECT OF CYTOKINES ON SPERM MOTILITY AND IONOPHORE-STIMULATED ACROSOME REACTION

被引：19
作者：
FEDDER, J [1 ]
ELLERMANNERIKSEN, S [1 ]
机构：
[1] AARHUS UNIV HOSP,DEPT GYNECOL & OBSTET,DK-8000 AARHUS,DENMARK
来源：
ARCHIVES OF ANDROLOGY | 1995年 / 35卷 / 03期
关键词：
ACROSOME REACTION; SPERM MOTILITY; CYTOKINES; REACTIVE OXYGEN SPECIES; LECTINS; HUMAN;
D O I：
10.3109/01485019508987870
中图分类号：
R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
摘要：
The effects of soluble products from leukocytes (WBC) on sperm motility and calcium ionophore-stimulated acrosome reaction (AR) were examined. Supernatants of mononuclear WBC, isolated from peripheral blood and stimulated by the lectins Phytolacca americana (pokeweed mitogen) or concanavalin A, caused a weak but significant inhibition of progressive sperm motility. The recombinant cytokine interferon-gamma (IFN-gamma) in high concentrations inhibited motility of sperm from 5 of 8 donors tested. The recombinant cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8) did not show any effect on sperm motility. Reactive oxygen species (ROS) produced by stimulation of polymorphonuclear WBC with a phorbol ester (PMA) tended to inhibit sperm motility. Neither supernatants from lectin-stimulated mononuclear WBC, IFN-gamma, TNF-alpha, IL-8, nor ROS showed any significant effect on the ionophore-challenged AR.
引用
收藏
页码：173 / 185
页数：13
相关论文
共 30 条
[1]   ANALYSIS OF THE RELATIONSHIP BETWEEN REACTIVE OXYGEN SPECIES PRODUCTION AND LEUKOCYTE INFILTRATION IN FRACTIONS OF HUMAN SEMEN SEPARATED ON PERCOLL GRADIENTS [J].
AITKEN, RJ ;
WEST, KM .
INTERNATIONAL JOURNAL OF ANDROLOGY, 1990, 13 (06) :433-451
[2]   PROSPECTIVE ANALYSIS OF SPERM-OOCYTE FUSION AND REACTIVE OXYGEN SPECIES GENERATION AS CRITERIA FOR THE DIAGNOSIS OF INFERTILITY [J].
AITKEN, RJ ;
IRVINE, DS ;
WU, FC .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1991, 164 (02) :542-551
[3]   GENERATION OF REACTIVE OXYGEN SPECIES, LIPID-PEROXIDATION, AND HUMAN-SPERM FUNCTION [J].
AITKEN, RJ ;
CLARKSON, JS ;
FISHEL, S .
BIOLOGY OF REPRODUCTION, 1989, 41 (01) :183-197
[4]  
BERKOW RL, 1987, J IMMUNOL, V139, P3783
[5]   EFFECTS OF PRODUCTS OF ACTIVATED LEUKOCYTES (LYMPHOKINES AND MONOKINES) ON THE GROWTH OF MALIGNANT TROPHOBLAST CELLS-INVITRO [J].
BERKOWITZ, RS ;
HILL, JA ;
KURTZ, CB ;
ANDERSON, DJ .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1988, 158 (01) :199-203
[6]   HYDROGEN-PEROXIDE IS INVOLVED IN HAMSTER SPERM CAPACITATION INVITRO [J].
BIZE, I ;
SANTANDER, G ;
CABELLO, P ;
DRISCOLL, D ;
SHARPE, C .
BIOLOGY OF REPRODUCTION, 1991, 44 (03) :398-403
[7]   ROLE OF THE CUMULUS IN THE SELECTION OF MORPHOLOGICALLY NORMAL SPERM AND INDUCTION OF THE ACROSOME REACTION DURING HUMAN IN-VITRO FERTILIZATION [J].
CARRELL, DT ;
MIDDLETON, RG ;
PETERSON, CM ;
JONES, KP ;
URRY, RL .
ARCHIVES OF ANDROLOGY, 1993, 31 (02) :133-137
[8]   INDUCTION OF ACROSOME REACTIONS BY THE HUMAN ZONA PELLUCIDA [J].
CROSS, NL ;
MORALES, P ;
OVERSTREET, JW ;
HANSON, FW .
BIOLOGY OF REPRODUCTION, 1988, 38 (01) :235-244
[9]   2 SIMPLE METHODS FOR DETECTING ACROSOME-REACTED HUMAN-SPERM [J].
CROSS, NL ;
MORALES, P ;
OVERSTREET, JW ;
HANSON, FW .
GAMETE RESEARCH, 1986, 15 (03) :213-226
[10]  
CUMMINS JM, 1991, J ANDROL, V12, P98
123