To determine the knowledge, attitudes and practices regarding eye donation in selected adult population attending tertiary eye care centre. Methods: 636 interviews were successfully completed and used for analysis. A questionnaire was specially designed to know the facts about awareness and willingness for eye donation among general population attending tertiary eye care centre. Questionnaire included whether they had any knowledge of eye donation, source of information, willingness to donate eyes and also impact of eye donation on corneal blindness status. Results: Knowledge about eye donation was significantly associated with education and socioeconomic status. After counseling, 84.90% of participants agreed to motivate other family members/friends to fill the pledge form of eye donation. Allowance of eye donation in religion was significantly associated with the motivation to donate. Higher level of education and higher socioeconomic status were significant predictors of knowledge status of eye donation. For motivation, higher socioeconomic status, adequate knowledge and belief that eye donation is allowed in religion were significant predictors. Television emerged as the major source of information. Conclusion: Better knowledge may ultimately translate into the act of donation. Effective measures should be taken to educate people with relevant information with the involvement of media, doctors and religious scholars.