INHIBITORY EFFECT OF OKADAIC ACID ON CARBACHOL-EVOKED SECRETION OF CATECHOLAMINES IN CULTURED BOVINE ADRENAL-MEDULLARY CELLS

被引：24
|
作者：
YANAGIHARA, N
TOYOHIRA, Y
KODA, Y
WADA, A
IZUMI, F
机构：
[1] Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 01期
关键词：
D O I：
10.1016/0006-291X(91)90487-R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We examined the effect of okadaic acid on catecholamine secretion caused by carbachol in cultured bovine adrenal medullary cells. Treatment of cells with 100 nM okadaic acid for 3-24 hr produced an inhibition of catecholamine secretion stimulated by carbachol. The half-maximal and maximal inhibition of secretion was observed at 40 nM and 300 nM okadaic acid for 24 hr, respectively. Okadaic acid also inhibited veratridine- and high K+-induced secretion but not ionomycin-induced secretion. Okadaic acid strongly suppressed 45Ca2+ influx and slightly inhibited 22Na+ influx in carbachol-stimulated cells. These results suggest that okadaic acid inhibits carbachol-evoked secretion of catecholamines mainly by suppression of Ca2+ influx in adrenal medullary cells. © 1991.
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页码：77 / 83
页数：7
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