CULTURE MICROTITRATION - A SENSITIVE METHOD FOR QUANTIFYING LEISHMANIA-INFANTUM IN TISSUES OF INFECTED MICE

被引:214
作者
BUFFET, PA [1 ]
SULAHIAN, A [1 ]
GARIN, YJF [1 ]
NASSAR, N [1 ]
DEROUIN, F [1 ]
机构
[1] FAC MED LARIBOISIERE,PARASITOL MYCOL LAB,F-75270 PARIS 06,FRANCE
关键词
D O I
10.1128/AAC.39.9.2167
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We developed a microtitration method to determine the parasite burdens in homogenized organs of mice infected with Leishmania infantum. This method proved more sensitive than direct enumeration of amastigotes in stained organs, was appropriate for describing the kinetics of infection, and can be considered for physiopathological or pharmaceutical experimental studies.
引用
收藏
页码:2167 / 2168
页数:2
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