MATURATION OF BACTERIOPHAGE P2-DNA INVITRO - COMPLEX, SITE-SPECIFIC SYSTEM FOR DNA CLEAVAGE

被引：28

作者：

BOWDEN, DW ^{[1
]}

CALENDAR, R ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT MOLEC BIOL,BERKELEY,CA 94720

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1979年 / 129卷 / 01期

关键词：

D O I：

10.1016/0022-2836(79)90055-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacteriophage P2-infected cell extracts lacking the M gene product can package linear (mature) P2 DNA, but not its closed circular precursor, into phage particles. Thus the P2 M gene product is required for site-specific DNA cleavage (ter cleavage) during DNA packaging. The P2 M and P gene products have been extensively purified using an in vitro complementation-packaging assay. When studied in vitro, the site-specific DNA cutting which generates mature P2 DNA requires P2 head structures in addition to purified M and P proteins, spermidine, and ATP. © 1979.

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页码：1 / 18

页数：18

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