MATURATION OF BACTERIOPHAGE P2-DNA INVITRO - COMPLEX, SITE-SPECIFIC SYSTEM FOR DNA CLEAVAGE

被引：28
作者：
BOWDEN, DW [1 ]
CALENDAR, R [1 ]
机构：
[1] UNIV CALIF BERKELEY,DEPT MOLEC BIOL,BERKELEY,CA 94720
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1979年 / 129卷 / 01期
关键词：
D O I：
10.1016/0022-2836(79)90055-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bacteriophage P2-infected cell extracts lacking the M gene product can package linear (mature) P2 DNA, but not its closed circular precursor, into phage particles. Thus the P2 M gene product is required for site-specific DNA cleavage (ter cleavage) during DNA packaging. The P2 M and P gene products have been extensively purified using an in vitro complementation-packaging assay. When studied in vitro, the site-specific DNA cutting which generates mature P2 DNA requires P2 head structures in addition to purified M and P proteins, spermidine, and ATP. © 1979.
引用
收藏
页码：1 / 18
页数：18
相关论文
共 41 条
[1]   INTERACTIONS BETWEEN A SATELLITE BACTERIOPHAGE AND ITS HELPER [J].
BARRETT, KJ ;
MARSH, ML ;
CALENDAR, R .
JOURNAL OF MOLECULAR BIOLOGY, 1976, 106 (03) :683-707
[2]   ENZYMATIC BREAKAGE OF COHESIVE END SITE OF PHAGE-LAMBDA DNA - TERMINASE (TER) REACTION [J].
BECKER, A ;
GOLD, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (09) :4199-4203
[3]   HOST CONTROLLED VARIATION IN BACTERIAL VIRUSES [J].
BERTANI, G ;
WEIGLE, JJ .
JOURNAL OF BACTERIOLOGY, 1953, 65 (02) :113-121
[4]   STUDIES ON LYSOGENESIS .1. THE MODE OF PHAGE LIBERATION BY LYSOGENIC ESCHERICHIA-COLI [J].
BERTANI, G .
JOURNAL OF BACTERIOLOGY, 1951, 62 (03) :293-300
[5]   PREPARATION AND CHARACTERIZATION OF TEMPERATE, NON-INDUCIBLE BACTERIOPHAGE P2 (HOST-ESCHERICHIA-COLI) [J].
BERTANI, LE ;
BERTANI, G .
JOURNAL OF GENERAL VIROLOGY, 1970, 6 :201-&
[6]   ABORTIVE INDUCTION OF BACTERIOPHAGE P2 [J].
BERTANI, LE .
VIROLOGY, 1968, 36 (01) :87-+
[7]   THE EFFECT OF THE INHIBITION OF PROTEIN SYNTHESIS ON THE ESTABLISHMENT OF LYSOGENY [J].
BERTANI, LE .
VIROLOGY, 1957, 4 (01) :53-71
[8]  
BOKLAGE CE, 1973, GENETICS, V75, P221
[9]   POSITION OF 2 DELETION MUTATIONS ON PHYSICAL MAP OF BACTERIOPHAGE P2 [J].
CHATTORAJ, DK ;
INMAN, RB .
JOURNAL OF MOLECULAR BIOLOGY, 1972, 66 (03) :423-+
[10]   RESTRICTION OF BACTERIOPHAGE-P2 BY ESCHERICHIA-COLI RI PLASMID, AND INVITRO CLEAVAGE OF ITS DNA BY ECORI ENDONUCLEASE [J].
CHATTORAJ, DK ;
OBEROI, YK ;
BERTANI, G .
VIROLOGY, 1977, 81 (02) :460-470
12345