THE NMR STRUCTURE OF THE INHIBITED CATALYTIC DOMAIN OF HUMAN STROMELYSIN-1

被引：132

作者：

GOOLEY, PR ^{[1
]}

OCONNELL, JF ^{[1
]}

MARCY, AI ^{[1
]}

CUCA, GC ^{[1
]}

SALOWE, SP ^{[1
]}

BUSH, BL ^{[1
]}

HERMES, JD ^{[1
]}

ESSER, CK ^{[1
]}

HAGMANN, WK ^{[1
]}

SPRINGER, JP ^{[1
]}

JOHNSON, BA ^{[1
]}

机构：

[1] MERCK & CO INC,MERCK SHARP & DOHME RES LABS,DEPT MED CHEM RES,RAHWAY,NJ 07065

来源：

NATURE STRUCTURAL BIOLOGY | 1994年 / 1卷 / 02期

关键词：

D O I：

10.1038/nsb0294-111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The three-dimensional structure of the catalytic domain of stromelysin-1 complexed with an N-carboxyl alkyl inhibitor has been determined by NMR methods. The global fold consists of three helices, a five stranded beta-sheet and a methionine located in a turn near the catalytic histidines, classifying stromelysin-1 as a metzincin. Stromelysin-1 is unique in having two independent zinc binding sites: a catalytic site and a structural site. The inhibitor binds in an extended conformation. The S1' subsite is a deep hydrophobic pocket, whereas S2' appears shallow and 53' open.

引用

页码：111 / 118

页数：8

共 51 条

[1] CRYSTAL-STRUCTURE AND N-14 NUCLEAR-QUADRUPOLE RESONANCE-SPECTRUM OF CATENA-MU-IMIDAZOLATO-BIS(IMIDAZOLE)ZINC NITRATE - DONOR CHARACTERISTICS OF COORDINATED IMIDAZOLATE [J].

ASHBY, CIH ;

CHENG, CP ;

DUESLER, EN ;

BROWN, TL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1978, 100 (19) :6063-6067

[2]

BERSTEIN FC, 1977, J MOL BIOL, V112, P535

[3] STRUCTURE OF ASTACIN AND IMPLICATIONS FOR ACTIVATION OF ASTACINS AND ZINC-LIGATION OF COLLAGENASES [J].

BODE, W ;

GOMISRUTH, FX ;

HUBER, R ;

ZWILLING, R ;

STOCKER, W .

NATURE, 1992, 358 (6382) :164-167

[4] ASTACINS, SERRALYSINS, SNAKE-VENOM AND MATRIX METALLOPROTEINASES EXHIBIT IDENTICAL ZINC-BINDING ENVIRONMENTS (HEXXHXXGXXH AND MET-TURN) AND TOPOLOGIES AND SHOULD BE GROUPED INTO A COMMON FAMILY, THE METZINCINS [J].

BODE, W ;

GOMISRUTH, FX ;

STOCKLER, W .

FEBS LETTERS, 1993, 331 (1-2) :134-140

[5] CHARMM - A PROGRAM FOR MACROMOLECULAR ENERGY, MINIMIZATION, AND DYNAMICS CALCULATIONS [J].

BROOKS, BR ;

BRUCCOLERI, RE ;

OLAFSON, BD ;

STATES, DJ ;

SWAMINATHAN, S ;

KARPLUS, M .

JOURNAL OF COMPUTATIONAL CHEMISTRY, 1983, 4 (02) :187-217

[6]

BRUNGER AT, 1992, X PLOR MANUAL VERSIO

[7] INHIBITION OF MATRIX METALLOPROTEINASES BY N-CARBOXYALKYL PEPTIDES [J].

CHAPMAN, KT ;

KOPKA, IE ;

DURETTE, PL ;

ESSER, CK ;

LANZA, TJ ;

IZQUIERDOMARTIN, M ;

NIEDZWIECKI, L ;

CHANG, B ;

HARRISON, RK ;

KUO, DW ;

LIN, TY ;

STEIN, RL ;

HAGMANN, WK .

JOURNAL OF MEDICINAL CHEMISTRY, 1993, 36 (26) :4293-4301

[8] 4-DIMENSIONAL C-13/C-13-EDITED NUCLEAR OVERHAUSER ENHANCEMENT SPECTROSCOPY OF A PROTEIN IN SOLUTION - APPLICATION TO INTERLEUKIN 1-BETA [J].

CLORE, GM ;

KAY, LE ;

BAX, A ;

GRONENBORN, AM .

BIOCHEMISTRY, 1991, 30 (01) :12-18

[9] STRUCTURE OF THERMOLYSIN - ELECTRON-DENSITY MAP AT 2.3 A RESOLUTION [J].

COLMAN, PM ;

MATTHEWS, BW ;

JANSONIUS, JN .

JOURNAL OF MOLECULAR BIOLOGY, 1972, 70 (03) :701-+

[10]

DUMERMUTH E, 1991, J BIOL CHEM, V266, P21381

← 1 2 3 4 5 6 →