DETECTION OF A NOVEL SEPIAPTERIN REDUCTASE MESSENGER-RNA - ASSAY OF MESSENGER-RNA IN VARIOUS CELLS AND TISSUES OF VARIOUS SPECIES

被引：6

作者：

MAIER, J

SCHOTT, K

WERNER, T

BACHER, A

ZIEGLER, I

机构：

[1] GSF UMWELT & GESUNDHEIT FORSCHUNGSZENTRUM,INST KLIN MOLEK BIOL & TUMORGENET,MARCHIONINSTR 25,W-8000 MUNICH 70,GERMANY

[2] GSF MUNICH,UMWELT & GESUNDHEIT FORSCHUNGSZENTRUM,INST SAUGETIERGENET,W-8042 NEUHERBERG,GERMANY

[3] TECH UNIV MUNICH,LEHRSTUHL ORGAN CHEM & BIOCHEM,W-8046 GARCHING,GERMANY

来源：

EXPERIMENTAL CELL RESEARCH | 1993年 / 204卷 / 02期

关键词：

D O I：

10.1006/excr.1993.1027

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Fragments of cDNA coding for rat, murine, and human sepiapterin reductase (SR) were amplified by PCR via primer positioning close to the reported 3′-end of the coding region in the rat enzyme. They were sequenced and used as probes for mRNA detection. Northern blot analysis detected two mRNA species for SR. Their sizes were 1.3 and 2.1 kb for rat, 1.3 and 2.3 kb for mouse, and 1.6 and 2.1 kb for human cell lines. Comparison of rat cell lines and rat tissues indicated that in tissues only the 1.3-kb species is present. Washing of the Northern blots under different stringency conditions indicated a more stable interaction of the 1.3-kb mRNA species with the cDNA probe as compared to the 2.3-kb species. The 1.3-kb species corresponds to the reported 28.2-kDa molecular mass of rat SR monomer. SR mRNA expression is absent in the human NK-like cell line YT and in the murine erythroleukemia subclone B8/3, which both lack SR activity. Moreover, the relative mRNA expression correlates with the enzymatic activities of different cell lines within the same species. This indicates that SR activity is regulated by its steady state mRNA levels. © 1993 Academic Press, Inc.

引用

页码：217 / 222

页数：6

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