MEASUREMENT OF HUMAN CHORIONIC-GONADOTROPIN DURING EARLY-PREGNANCY - A COMPARISON OF 2 IMMUNORADIOMETRIC ASSAYS

被引：0

作者：

MATSON, PL

NEWMAN, MC

MORROLL, D

TROUP, SA

LIEBERMAN, BA

机构：

[1] Regional IVF Unit, St. Mary's Hospital, Manchester, M13 0JH, Whitworth Park

[2] Manchester Fertility Services, Manchester BUPA Hospital, Manchester, M16 8AJ, Russell Road

来源：

JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER | 1990年 / 7卷 / 03期

关键词：

gamete intrafallopian transfer; human chorionic gonadotropin; in vitro fertilization;

D O I：

10.1007/BF01135683

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Evidence of implantation following either in vitro fertilization and embryo transfer (IVF-ET) or gamete intrafallopian transfer (GIFT) was obtained by collecting blood on days 10, 12, 14, 16, and 18 after oocyte recovery (day 0), and retrospectively measuring human chorionic gonadotropin (hCG). This was done using immunoradiometric assays for hCG, manufactured either by Serono Diagnostics Ltd. (MAIAclone) or Diagnostics Products Corporation (IRMA-count). The analysis of 63 serum samples by both kits showed a good correlation (r=0.99) but the Serono (y) method gave values which were consistently greater (y=1.58x+4.89) than those of the DPC (x) method. A comparison of the hCG profile of singleton pregnancies measured with either the Serono (n =33) or DPC (n=22) kits gave a similar relationship. These results suggest that great care must be taken when comparing results from different laboratories using different kits. Also, consideration must be given to the possible loss of a continuous database should a laboratory change kit. © 1990 Plenum Publishing Corporation.

引用

页码：168 / 171

页数：4

共 13 条

[1]

Grudzinskas J.G., Westergaard J.G., Teisner B., Biochemical assessment of placental function: Early pregnancy, Clin Obstet Gynaecol, 13, pp. 553-569, (1986)

[2]

Mitchell H.D.C., Bagshawe K.D., Human chorionic gonatrophin, Hormone Assays and Their Clinical Application, (1976)

[3]

Yamashita T., Okamoto S., Thomas A., McLachlan V., Healy D.L., Predicting pregnancy outcome after in vitro fertilization and embryo transfer using oestradiol, progesterone, and human chorionic gonadotropin β-subunit, Fertil Steril, 51, pp. 304-309, (1989)

[4]

Englert Y., Roger M., Belaisch-Allart J., Jondet M., Frydman R., Testart J., Delayed appearance of plasmatic chorionic gonadotropin in pregnancies after in vitro fertilisation and embryo transfer, Fertil Steril, 42, pp. 835-838, (1984)

[5]

Remorgida V., Anserini P., Costa M., Gaggero G., Groce S., Capitanio G.L., Gamete intrafallopian transfer and plasma chorionic gonadotropin dynamics: Evidence of a delayed appearance, Journal of In Vitro Fertilization and Embryo Transfer, 5, pp. 195-198, (1988)

[6]

Hay D.L., Discordant and variable production of human chorinic gonadotrophin and its free α- and β-subunits in early pregnancy, J Clin Endocrinol Metab, 61, pp. 1195-1200, (1985)

[7]

Heip J., Liu Y., Devroey P., Naaktgeboren N., Van Steirteghem A.C., Evaluation of radiommunoassays using mono- and polyclonal antibodies for the quantitation of human chorionic gonadotrophin in early pregnancy, Ann Biol Clin (Paris), 44, pp. 131-138, (1986)

[8]

Jones H.W., Acosta A.A., Andrews M.C., Garcia J.E., Jones G.S., Mantzavinos T., McDowell J., Sandow B.A., Veeck L., Whibley T.W., Wilkes C.A., Wright G.L., What is a pregnancy? A question for programs of in vitro fertilization, Fertil Steril, 40, pp. 728-733, (1983)

[9]

Confino E., Demir R.H., Friberg J., Gleicher N., The predictive value of hCG beta subunit levels in pregnancies achieved by in vitro fertilisation and embryo transfer: An international collaborative study, Fertil Steril, 45, pp. 526-531, (1986)

[10]

Batzen F.R., Schloff S., Goldfarb A.F., Corson S.L., Serial β-subunit human chorionic gonadotropin doubling time as a prognostic indicator of pregnancy outcome in an infertile population, Fertil Steril, 35, pp. 307-312, (1981)

← 1 2 →