PHENYLALANINE HYDROXYLASE OF HUMAN LIVER - ASSAY AND SOME PROPERTIES

被引：61
作者：
KAUFMAN, S
机构：
[1] Laboratory of Neurochemistry, National Institute of Mental Health, Bethesda
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 134卷 / 01期
关键词：
D O I：
10.1016/0003-9861(69)90273-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive, reliable assay for phenylalanine hydroxylase in human liver extracts is described. A few milligrams of tissue are sufficient for a quantitative determination of the enzyme activity in normal liver. With this procedure, the apparent Km values for phenylalanine and for 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, a cofactor analogue, have been determined for the human liver enzyme. © 1969.
引用
收藏
页码：249 / &
相关论文
共 13 条
[1]   ATYPICAL PHENYLKETONURIC HETEROZYGOTE [J].
ANDERSON, JA ;
FISCH, R ;
MILLER, E ;
DOEDEN, D .
JOURNAL OF PEDIATRICS, 1966, 68 (03) :351-+
[2]   A SIMPLE EFFICIENT LIQUID SCINTILLATOR FOR COUNTING AQUEOUS SOLUTIONS IN A LIQUID SCINTILLATION COUNTER [J].
BRAY, GA .
ANALYTICAL BIOCHEMISTRY, 1960, 1 (4-5) :279-285
[3]  
JUSTICE P, 1967, LANCET, V1, P928
[4]  
KAUFMAN S, 1959, J BIOL CHEM, V234, P2677
[5]  
KAUFMAN S, 1959, J BIOL CHEM, V234, P2683
[6]  
KAUFMAN S, 1957, J BIOL CHEM, V226, P511
[7]  
KAUFMAN S, 1967, PHENYLKETONURIA ALLI, P205
[8]  
KAUFMAN S, METHODS ENZYMOLOGY A, V17
[9]  
Kaufmann S., 1962, OXYGENASES, P129
[10]  
LADU BN, 1967, PHENYLKETONURIA ALLI, P193
12