DEGRADATION OF PHOTOSYSTEM-II REACTION-CENTER D1-PROTEIN INDUCED BY UVB RADIATION IN ISOLATED THYLAKOIDS - IDENTIFICATION AND CHARACTERIZATION OF C-TERMINAL AND N-TERMINAL BREAKDOWN PRODUCTS

被引：94
作者：
FRISO, G
SPETEA, C
GIACOMETTI, GM
VASS, I
BARBATO, R
机构：
[1] UNIV PADUA,DEPT BIOL,I-35121 PADUA,ITALY
[2] BIOL RES CTR,INST PLANT PHYSIOL,H-6701 SZEGED,HUNGARY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1994年 / 1184卷 / 01期
关键词：
UVB; PHOTOINHIBITION; PHOTOSYSTEM II; D1-PROTEIN; THYLAKOID;
D O I：
10.1016/0005-2728(94)90156-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of UVB radiation on the stability of Photosystem II reaction center D1-protein in isolated thylakoids are investigated. A new C-terminal degradation product has been identified and characterized, produced by cleavage in the second transmembrane segment. Immunological evidence for the presence of N-terminal fragments corresponding to the remaining part of D1-protein is also provided. The appearance of these fragments is not affected by lowering the temperature from 22 degrees C to 4 degrees C, changing the pH from 6 to 8, adding soybean trypsin inhibitor or excluding oxygen from the thylakoid suspension.
引用
收藏
页码：78 / 84
页数：7
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