TRANSFERRIN CAN REPLACE SERUM FOR INVITRO GROWTH OF MITOGEN-STIMULATED LYMPHOCYTES-T

被引：86

作者：

DILLNERCENTERLIND, ML

HAMMARSTROM, S

PERLMANN, P

机构：

[1] Department of Immunology, University of Stockholm, Stockholm

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1979年 / 9卷 / 12期

关键词：

D O I：

10.1002/eji.1830091207

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Activation of human T cells by mitogens was compared in cultures containing serum, human serum albumin or purified human transferrin as growth support. The mitogenic effect of the lectins leucoagglutinin, concanavalin A and Wistaria floribunda agglutinin was measured as incorporation of [3H]thymidine into the cellular DNA of the lymphocytes. Three different preparations of transferrin were all able to fully substitute serum or serum albumin as growth promotors, when present at concentrations of 10 μg/ml or more. A small contamination of transferrin in the human serum albumin preparations used was shown to be responsible for their growth‐supporting effect, while no need for the presence of albumin itself could be demonstrated. Copyright © 1979 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：942 / 948

页数：7

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