FORMATION OF ACYLPHOSPHATIDYLGLYCEROL BY A LYSOSOMAL PHOSPHATIDYLCHOLINE - BIS(MONOACYLGLYCERO)PHOSPHATE ACYL TRANSFERASE

被引:11
作者
HUTERER, SJ [1 ]
WHERRETT, JR [1 ]
机构
[1] UNIV TORONTO,DEPT MED NEUROL,6368 MED SCI BLDG,TORONTO M5G 1A8,ONTARIO,CANADA
来源
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1990年 / 68卷 / 01期
关键词
D O I
10.1139/o90-050
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A delipidated soluble fraction prepared from a mitochondrial-lysosomal fraction of rabbit alveolar macrophages that catalyzes transacylation of lysophosphatidylglycerol to form bis(monoacylglycero)phosphate was also found to transfer oleic acid from [14C]dioleoyl phosphatidylcholine to form acylphosphatidylglycerol. The reaction was dependent on the presence of bis(monoacylglycero)phosphate and was maximal at a concentration of 44 μM when the ratio of fatty acid transferred to fatty acid released was 0.28. Addition of phosphatidylglycerol had only a small effect. Homogenates of rat liver also catalyzed the reaction and after subcellular fractionation the activity was localized to lysosomes. The lysosomal activity was solubilized by delipidation with butanol to give a preparation with a specific activity 2462 times that of the homogenate. Optimal activity of soluble preparations from both macrophages and liver was at pH 4.5, with little activity above 6.0. Release of free fatty acid was also stimulated under conditions of optimal acyl transfer. Both acyl transfer and release of fatty acid were inhibited by Ca2+, detergents, chloropromazine, lysophosphatidylcholine, and oleic acid. Where there was disproportional inhibition, acyl transfer was always more affected. These results suggest that sequential acylation of lysophosphatidylglycerol to form bis(monoacylglycero)phosphate and then acylphosphatidylglycerol constitute a mechanism in the lysosome for the transport and partition of fatty acids released by the lysosomal phospholipases.
引用
收藏
页码:366 / 372
页数:7
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