ALPHA-GLUCURONIDASE IN 2 MICROBIAL XYLANOLYTIC SYSTEMS

被引：87

作者：

PULS, J ^{[1
]}

SCHMIDT, O ^{[1
]}

GRANZOW, C ^{[1
]}

机构：

[1] BUNDESFORSCH ANSTALT FORST & HOLZWIRTSCHAFT, INST HOLZCHEM & CHEM TECHNOL HOLZES, D-2050 HAMBURG 80, FED REP GER

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1987年 / 9卷 / 02期

关键词：

D O I：

10.1016/0141-0229(87)90147-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The mode of action and some properties of the newly discovered fungal enzyme .alpha.-glucoronidase are described. The enzyme acts synergistically with xylanases and liberates 4-O-methyylglucuronic acid from 4-O-methylglucuronic acid-substituted xyloogligomers. .alpha.-Glucuronidase is readily produced by Agaricus bisporus and Pleurotus ostreatus, which are, however, poor producers of xylanases and .beta.-xylosidases. Comparative fermentations with different media showed (4-O-methylglucurono)-D-xylan from beechwood to be the best substrate and A. bisporus to be the more powderful organism. An enzyme test for .alpha.-glucuronidase activity has been developed. 2-O-(4-O-Methyl-.alpha.-D-glucopyranosyluronic acid)-D-xylobiose, which is neither degraded by xylanases nor by .beta.-xylosidases, was used as substrate. The uronic acid substituent was bound by an .alpha.-1,2 linkage to the non-reducing xylose residue. Anion exchange chromatography in acetate medium was a powerful tool for detection of degradation products.

引用

页码：83 / 88

页数：6

共 20 条

[1] ACETYL XYLAN ESTERASES IN FUNGAL CELLULOLYTIC SYSTEMS [J].