TEMPERATURE-DEPENDENCE OF PROTEIN SOLUBILITY - DETERMINATION AND APPLICATION TO CRYSTALLIZATION IN X-RAY CAPILLARIES

被引：143
|
作者：
ROSENBERGER, F
HOWARD, SB
SOWERS, JW
NYCE, TA
机构：
[1] Center for Microgravity and Materials Research, University of Alabama in Huntsville, Huntsville
来源：
JOURNAL OF CRYSTAL GROWTH | 1993年 / 129卷 / 1-2期
基金：
美国国家科学基金会; 美国国家航空航天局;
关键词：
D O I：
10.1016/0022-0248(93)90427-X
中图分类号：
O7 [晶体学];
学科分类号：
0702 ; 070205 ; 0703 ; 080501 ;
摘要：
A scintillation method was developed for determinations of the temperature dependence of the solubility, and of nucleation induction times of proteins, in 50-100 mul volumes of solution. Solubility data for lysozyme and horse serum albumin were obtained for various combinations of pH and precipitant concentrations. These data and the nucleation induction information were used for dynamic crystallization control, that is, for the controlled separation of nucleation and growth stages. Individual lysozyme and horse serum albumin crystals were grown in 15-20 mul solution volumes contained in X-ray capillaries.
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页码：1 / 12
页数：12
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