PLEIOTROPIC EFFECTS OF TOBACCO-MOSAIC-VIRUS MOVEMENT PROTEIN ON CARBON METABOLISM IN TRANSGENIC TOBACCO PLANTS

被引:2
作者
OLESINSKI, AA
LUCAS, WJ
GALUN, E
WOLF, S
机构
[1] HEBREW UNIV JERUSALEM,FAC AGR,DEPT VEGETABLE CROPS,IL-76100 REHOVOT,ISRAEL
[2] UNIV CALIF DAVIS,DIV BIOL SCI,PLANT BIOL SECT,DAVIS,CA 95616
[3] WEIZMANN INST SCI,DEPT PLANT GENET,IL-76100 REHOVOT,ISRAEL
关键词
CARBON(ALLOCATION; METABOLISM); MOVEMENT PROTEIN; NICOTIANA; PLASMODESMA; TOBACCO MOSAIC VIRUS; TRANSGENIC TOBACCO;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) plants expressing wild-type or mutant forms of the 30-kDa movement protein of tobacco mosaic virus (TMV-MP) were employed to study the effects of the TMV-MP on carbon metabolism in source leaves. Fully expanded source leaves of transgenic plants expressing the TMV-MP were found to retain more newly fixed C-14 compared with control plants. Analysis of C-14-export from young leaves of TMV-MP plants, where the MP is yet to influence plasmodesmal size exclusion limit, indicated a similar pattern, in that daytime C-14 export was slower in TMV-MP plants as compared to equivalent-aged leaves on control plants. Pulse-chase experiments were used to monitor radioactivity present in the different carbohydrate fractions, at specified intervals following (CO2)-C-14 labeling. These studies established that the TMV-MP can cause a significant adjustment in short-term C-14-photosynthate storage and export. That these effects of the TMV-MP on carbon metabolism and phloem function were not attributable to the effect of this protein on plasmodesmal size exclusion limits, per se, was established using transgenic tobacco plants expressing temperature-sensitive and C-terminal deletion mutant forms of the TMV-MP. Collectively, these studies establish the pleiotropic nature of the TMV-MP in transgenic tobacco, and the results are discussed in terms of potential sites of interaction between the TMV-MP and endogenous processes involved in regulating carbon metabolism and export.
引用
收藏
页码:118 / 126
页数:9
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