GENETIC EXCHANGE IN TRYPANOSOMA-BRUCEI - SELECTION OF HYBRID TRYPANOSOMES BY INTRODUCTION OF GENES CONFERRING DRUG-RESISTANCE

被引：31
作者：
GIBSON, W
WHITTINGTON, H
机构：
[1] Department of Pathology and Microbiology, University of Bristol Veterinary School, Langford, Bristol
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1993年 / 60卷 / 01期
基金：
英国医学研究理事会;
关键词：
TRYPANOSOME; TRYPANOSOMA-BRUCEI; GENETIC EXCHANGE; TRANSFORMATION;
D O I：
10.1016/0166-6851(93)90024-R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Genetic exchange in Trypanosoma brucei ssp. can occur when 2 different strains are cotransmitted through the tsetse fly vector. We have introduced heterologous genes for drug resistance (neo or hph) into parental trypanosome lines by electroporation. Drug resistant lines were then crossed in vivo in the fly or in vitro. Hybrids were subsequently selected by double drug resistance. Analysis of trypanosomes from both fly midguts and salivary glands showed the latter to be the probable site of genetic exchange. This is one of the first applications of reverse genetics to a longstanding problem in parasite biology.
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页码：19 / 26
页数：8
相关论文
共 28 条
[1]   ACTIVATION OF TRYPANOSOME SURFACE GLYCOPROTEIN GENES INVOLVES A DUPLICATION-TRANSPOSITION LEADING TO AN ALTERED 3' END [J].
BERNARDS, A ;
VANDERPLOEG, LHT ;
FRASCH, ACC ;
BORST, P ;
BOOTHROYD, JC ;
COLEMAN, S ;
CROSS, GAM .
CELL, 1981, 27 (03) :497-505
[2]   NEW CULTURE MEDIUM FOR MAINTENANCE OF TSETSE TISSUES AND GROWTH OF TRYPANOSOMATIDS [J].
CUNNINGHAM, I .
JOURNAL OF PROTOZOOLOGY, 1977, 24 (02) :325-329
[3]   STABLE INTEGRATIVE TRANSFORMATION OF TRYPANOSOMA-BRUCEI THAT OCCURS EXCLUSIVELY BY HOMOLOGOUS RECOMBINATION [J].
EID, J ;
SOLLNERWEBB, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2118-2121
[4]   GENETIC EXCHANGE IN TRYPANOSOMA-BRUCEI-BRUCEI - VARIABLE CHROMOSOMAL LOCATION OF HOUSEKEEPING GENES IN DIFFERENT TRYPANOSOME STOCKS [J].
GIBSON, W ;
GARSIDE, L .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 45 (01) :77-90
[5]  
GIBSON W, 1992, MOL BIOCHEM PARASIT, V52, P189
[6]   STABLE INTRODUCTION OF EXOGENOUS DNA INTO TRYPANOSOMA-BRUCEI [J].
GIBSON, WC ;
WHITE, TC ;
LAIRD, PW ;
BORST, P .
EMBO JOURNAL, 1987, 6 (08) :2457-2461
[7]   ANALYSIS OF A GENETIC CROSS BETWEEN TRYPANOSOMA-BRUCEI-RHODESIENSE AND TRYPANOSOMA-BRUCEI-BRUCEI [J].
GIBSON, WC .
PARASITOLOGY, 1989, 99 :391-402
[8]   SPECIES-SPECIFIC DNA PROBES FOR THE IDENTIFICATION OF AFRICAN TRYPANOSOMES IN TSETSE FLIES [J].
GIBSON, WC ;
DUKES, P ;
GASHUMBA, JK .
PARASITOLOGY, 1988, 97 :63-73
[9]   CONTINUOUS CULTIVATION OF TRYPANOSOMA-BRUCEI BLOOD STREAM FORMS IN A MEDIUM CONTAINING A LOW CONCENTRATION OF SERUM-PROTEIN WITHOUT FEEDER CELL-LAYERS [J].
HIRUMI, H ;
HIRUMI, K .
JOURNAL OF PARASITOLOGY, 1989, 75 (06) :985-989
[10]   HYBRID FORMATION BETWEEN AFRICAN TRYPANOSOMES DURING CYCLICAL TRANSMISSION [J].
JENNI, L ;
MARTI, S ;
SCHWEIZER, J ;
BETSCHART, B ;
LEPAGE, RWF ;
WELLS, JM ;
TAIT, A ;
PAINDAVOINE, P ;
PAYS, E ;
STEINERT, M .
NATURE, 1986, 322 (6075) :173-175
123