THE DEDUCED AMINO-ACID-SEQUENCE OF HUMAN CARBONIC ANHYDRASE-RELATED PROTEIN (CARP) IS 98-PERCENT IDENTICAL TO THE MOUSE HOMOLOG

被引：35

作者：

SKAGGS, LA ^{[1
]}

BERGENHEM, NCH ^{[1
]}

VENTA, PJ ^{[1
]}

TASHIAN, RE ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT HUMAN GENET,ANN ARBOR,MI 48109

来源：

GENE | 1993年 / 126卷 / 02期

关键词：

PCR; POLYMERASE CHAIN REACTION; EVOLUTION; ISOZYMES;

D O I：

10.1016/0378-1119(93)90385-G

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A recently reported mRNA, encoding 'carbonic anhydrase-related polypeptide' (CARP) from the Purkinje cells of mouse cerebellum, was shown to have a 30-40% deduced amino acid sequence identity with the carbonic anhydrases (CA) of mammals. In order to compare the mouse and human CARP sequences, we used the polymerase chain reaction (PCR) to amplify human CARP sequences from several cDNA libraries (salivary gland, testis and placenta). The sequence has an 89.3% sequence identity with mouse CARP at the nucleotide level and 97.9% at the amino acid level. This extremely high evolutionary conservation suggests an important function for the CARP gene product.

引用

页码：291 / 292

页数：2

共 7 条

[1] IMPROVEMENT OF PCR AMPLIFIED DNA SEQUENCING WITH THE AID OF DETERGENTS [J].