THE TBP-TFIIA INTERACTION IN THE RESPONSE TO ACIDIC ACTIVATORS IN-VIVO

被引：105

作者：

STARGELL, LA ^{[1
]}

STRUHL, K ^{[1
]}

机构：

[1] HARVARD UNIV, SCH MED, DEPT BIOL CHEM & MOLEC PHARMACOL, BOSTON, MA 02115 USA

来源：

SCIENCE | 1995年 / 269卷 / 5220期

关键词：

D O I：

10.1126/science.7604282

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A yeast TBP mutant (N2-1) is described here that is defective specifically in responding to acidic activators in vivo. N2-1 does not support activation by Gal4, Ace1, and Gcn4, but appears unaffected for constitutive transcription, repression by the Cyc8-Tup1 and Not complexes, and transcription by polymerase I (Pol) and Pol III. In vitro, N2-1 fails to interact with TFIIA, but it associates normally with a TATA element, an acidic activation domain, and TFIIB. Fusion of the small subunit of TFIIA to N2-1 restores activation function in vivo. Thus, an efficient interaction between TBP and TFIIA is required for transcriptional activation in vivo.

引用

页码：75 / 78

页数：4

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