NUCLEOPHILIC CATALYSTS IN H-PHOSPHONATE INTERNUCLEOTIDE CONDENSATION REACTION

被引：0

作者：

DUBEY, IY ^{[1
]}

EFIMOV, VA ^{[1
]}

LYAPINA, TV ^{[1
]}

FEDORYAK, DM ^{[1
]}

机构：

[1] MM SHEMYAKIN BIOORGAN CHEM INST,MOSCOW 117312,USSR

来源：

BIOORGANICHESKAYA KHIMIYA | 1992年 / 18卷 / 07期

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摘要：

The mechanism of the H-phosphonate internucleotide condensation in the presence of different bases has been investigated by means of P-31 NMR spectroscopy and kinetic studies. Pyridine and quinoline are shown to exert nucleophilic catalysis at the step of the phosphorylation of nucleoside component by mixed anhydride. The activation of P-component by pivaloyl chloride in the presence of more powerful nucleophylic catalysts, such as Melm, DMAP, pyridine N-oxides, lead to rapid formation of acylphosphonate-base complexes, which display low phosphorylating activity.

引用

页码：911 / 919

页数：9