LACTOBACILLUS-KEFIR ALCOHOL-DEHYDROGENASE - A USEFUL CATALYST FOR SYNTHESIS

被引：197
作者：
BRADSHAW, CW
HUMMEL, W
WONG, CH
机构：
[1] Scripps Res Inst, RES INST, DEPT CHEM, 10666 N TORREY PINES RD, LA JOLLA, CA 92037 USA
[2] UNIV DUSSELDORF, INST ENZYMTECHNOL, W-4000 DUSSELDORF 1, GERMANY
[3] FORSCHUNGSZENTRUM JULICH, FORSCHUNGSZENTRUM JULICH, W-5170 JULICH 1, GERMANY
来源：
JOURNAL OF ORGANIC CHEMISTRY | 1992年 / 57卷 / 05期
关键词：
D O I：
10.1021/jo00031a037
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
The alcohol dehydrogenase from Lactobacillus kefir simultaneously catalyzes carbonyl reductions and NADPH regeneration in the presence of 2-propanol. Representative synthesis of a number of chiral alcohols was accomplished in good yield and high enantiomeric excess (94-99%). This NADPH-requiring enzyme transfers the pro-R hydride from the cofactor to the si face of carbonyls to give (R) alcohols. The enzyme exhibits a very broad substrate specificity and high enantioselectivity for the synthesis of chiral aromatic, cyclic, polycyclic, and aliphatic alcohols.
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页码：1532 / 1536
页数：5
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