A GENETIC-LOCUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI NECESSARY FOR THE PRODUCTION OF ATTACHING AND EFFACING LESIONS ON TISSUE-CULTURE CELLS

被引：1023

作者：

JERSE, AE

YU, J

TALL, BD

KAPER, JB

机构：

[1] UNIV MARYLAND, SCH MED, DEPT MED, DIV GEOG MED, CTR VACCINE DEV, BALTIMORE, MD 21201 USA

[2] UNIV MARYLAND, SCH MED, DEPT MICROBIOL & IMMUNOL, BALTIMORE, MD 21201 USA

[3] MED BIOTECHNOL CTR, BALTIMORE, MD 21201 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 20期

关键词：

Bacterial adhesion; Bacterial pathogenesis; Diarrhea;

D O I：

10.1073/pnas.87.20.7839

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The ability of enteropathogenic Escherichia coli (EPEC) to form attaching and effacing intestinal lesions is a major characteristic of EPEC pathogenesis. Using TnphoA mutagenesis we have identified a chromosomal gene (eae, for E. coli attaching and effacing) that is necessary for this activity. A DNA probe derived from this gene hybridizes to 100% of E. coli of EPEC serogroups that demonstrate attaching and effacing activity on tissue culture cells as well as other pathogenic E. coli that produce attaching and effacing intestinal lesions, such as RDEC-1 (an EPEC of weanling rabbits) and enterohemorrhagic E. coli. The predicted amino acid sequence derived from the nucleotide sequence of eae shows significant homology to that of the invasin of Yersinia pseudotuberculosis.

引用

页码：7839 / 7843

页数：5

共 44 条

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