HIGH-LEVEL EXPRESSION OF STREPTOKINASE IN ESCHERICHIA-COLI

被引：48

作者：

ESTRADA, MP

HERNANDEZ, L

PEREZ, A

RODRIGUEZ, P

SERRANO, R

RUBIERA, R

PEDRAZA, A

PADRON, G

ANTUCH, W

DELAFUENTE, J

HERRERA, L

机构：

[1] CTR INGN GENET & BIOTECNOL, DIV MAMMALIAN CELL GENET, POB 6162, HAVANA 6, CUBA

[2] CTR INGN GENET & BIOTECHNOL, ANALYT UNIT, HAVANA 6, CUBA

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 10期

关键词：

D O I：

10.1038/nbt1092-1138

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Streptokinase (SK), which activates human plasminogen by promoting its conversion to plasmin, is normally obtained from beta-hemolytic streptococci. Treatment with SK is an effective therapy for improving survival and preserving left ventricular function after coronary thrombosis. We report the cloning, expression in E. coli to levels of 25% of the total cell protein, and characterization of a novel SK (SKC-2) gene, the product of which is functionally equivalent to the naturally-derived protein. The availability of a recombinant streptokinase (rSK) in high yield and purity offers a potentially attractive alternative source of this important therapeutic agent.

引用

页码：1138 / 1142

页数：5

共 38 条

[1]

[Anonymous], 1987, Lancet, V2, P871

[2]

[Anonymous], 1988, LANCET, V2, P349

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