ANTIBODIES DIRECTED AGAINST HAEMOPHILUS-DUCREYI HEAT-SHOCK PROTEINS

被引：9

作者：

BROWN, TJ ^{[1
]}

JARDINE, J ^{[1
]}

ISON, CA ^{[1
]}

机构：

[1] ST MARYS HOSP, SCH MED, DEPT MED MICROBIOL, NORFOLK PL, LONDON W2 1PG, ENGLAND

来源：

MICROBIAL PATHOGENESIS | 1993年 / 15卷 / 02期

关键词：

HEAT SHOCK PROTEINS; HAEMOPHILUS-DUCREYI;

D O I：

10.1006/mpat.1993.1063

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The response to heat shock in Haemophilus ducreyi has been investigated by labelling newly synthesized proteins with a pulse of 35S-methionine at a range of temperatures between 30-40°C. Proteins were then separated by SDS-PAGE and analysed by autoradiography of the resultant profiles. Four heat shock proteins (HSP) were identified of apparent molecular weight <14, 58.5, 74 and 78 kDa. The three larger proteins appeared to be independently controlled and inducible in a range of H. ducreyi strains. The protein profiles were also probed by Western blot with serum from an immunized rabbit, serum from a rabbit infected intradermally with H. ducreyi, mouse monoclonal antibodies (MAb) and a pool of human sera from patients with chancroid. The rabbit sera, the human serum pool and MAb (BB11) reacted with epitopes on the HSP of 58.5 kDa, whereas only the immunized rabbit serum and another MAb (CC11) reacted with epitopes on the HSP of 78 kDa. The HSP of 58.5 kDa appears to be immunogenic and may play a part in the host response to H. ducreyi and the pathogenesis of chancroid. © 1993 Academic Press.

引用

页码：131 / 139

页数：9

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