3 ADDITIONAL GENES INVOLVED IN PYRIMIDINE DIMER REMOVAL IN SACCHAROMYCES-CEREVISIAE - RAD7, RAD14 AND MMS19

被引：65

作者：

PRAKASH, L ^{[1
]}

PRAKASH, S ^{[1
]}

机构：

[1] UNIV ROCHESTER,DEPT BIOL,ROCHESTER,NY 14627

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 176卷 / 03期

关键词：

D O I：

10.1007/BF00333097

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability to remove ultraviolet (UV)-induced pyrimidine dimers from the nuclear DNA of yeast was examined in two radiation-sensitive (rad) mutants and one methyl methanesulfonate-sensitive (mms) mutant of the yeast Saccharomyces cerevisiae. The susceptibility of DNA from irradiated cells to nicking by an endonuclease activity prepared from crude extracts of Micrococcus luteus was used to measure the presence of dimers in DNA. The rad7, rad14 and mms19 mutants were found to be defective in their ability to remove UV-induced dimers from nuclear DNA. All three mutants belong to the same epistatic group as the other mutants involved in excision-repair. All three mutants show enhanced UV-induced mutations. The rad14 mutant also shows epistatic interactions with genes in the other two UV repair pathways. © 1979 Springer-Verlag.

引用

页码：351 / 359

页数：9

共 30 条

[1] 4 RADIATION SENSITIVE MUTANTS OF SACCHAROMYCES - SURVIVAL AFTER UV-IRRADIATION AND X-RAY-IRRADIATION AS WELL AS UV-INDUCED REVERSION RATES FROM ISOLEUCINE-VALINE DEPENDENCE TO INDEPENDENCE [J].