EXAMINATION OF POLY(U)-DEPENDENT BINDING OF TRNA(PHE) TO RIBOSOMAL P-SITE BY NITROSOETHYLUREA MODIFICATION

被引：0
|
作者：
NEKHAI, SA
SAMINSKII, EM
机构：
来源：
MOLECULAR BIOLOGY | 1994年 / 28卷 / 04期
关键词：
TRNA(PHE); RIBOSOME; NITROSOETHYLUREA MODIFICATION;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A study was made of nitrosoethylurea alkylation of tRNA(Phe) phosphates bound to the P site of E. coli 70S ribosomes and 30S subunits. Upon binding of tRNA to the 30S subunit, the reagent was not attached to nucleotides 23-44, i.e., to the anticodon loop and the neighboring region of the D stem. Upon binding to the P site of 70S ribosomes, nucleotides 45-49, 51, 54-55, 58-61, and 63 (T loop) were protected. The results obtained indicate that nitrosoethylurea can be used for studying the tRNA-ribosome interaction.
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页码：604 / 607
页数：4
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