MINICELL-FORMING MUTANTS OF ESCHERICHIA-COLI - SUPPRESSION OF BOTH DICB-DEPENDENT AND MIND-DEPENDENT DIVISION INHIBITION BY INACTIVATION OF THE MINC GENE-PRODUCT

被引：41

作者：

LABIE, C ^{[1
]}

BOUCHE, F ^{[1
]}

BOUCHE, JP ^{[1
]}

机构：

[1] CNRS,CTR RECH BIOCHIM & GENET CELLULAIRES,118 ROUTE NARBONNE,F-31062 TOULOUSE,FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 10期

关键词：

D O I：

10.1128/jb.172.10.5852-5855.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have determined the nucleotide sequence of the minB operon of 10 min mutants of Escherichia coli, characterized by impaired inhibition of polar divisions. These mutants were either sensitive or resistant to the division inhibitor DicB. All the mutations were found to lie in minC or minD, confirming the requirement of both gene products in the process of inhibition of polar sites. Mutations conferring resistance to inhibitor DicB were found exclusively in minC. In agreement with de Boer et al. (P.A.J. de Boer, R.E. Crosseley, and L.I. Rothfield, Proc. Natl. Acad. Sci. USA 87:1129-1133, 1990), these results provide evidence that, in addition to promoting division inhibition with MinD, protein MinC acts in concert with DicB to inhibit division by a second, MinD-independent process.

引用

页码：5852 / 5855

页数：4

共 17 条

[1] MINIATURE ESCHERICHIA COLI CELLS DEFICIENT IN DNA [J].

ADLER, HI ;

FISHER, WD ;

COHEN, A ;

HARDIGREE, AA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 57 (02) :321-+

[2] IDENTIFICATION AND SEQUENCE OF GENE DICB - TRANSLATION OF THE DIVISION INHIBITOR FROM AN IN-PHASE INTERNAL START [J].

CAM, K ;

BEJAR, S ;

GIL, D ;

BOUCHE, JP .

NUCLEIC ACIDS RESEARCH, 1988, 16 (14) :6327-6338

[3] A PSC101-DERIVED PLASMID WHICH SHOWS NO SEQUENCE HOMOLOGY TO OTHER COMMONLY USED CLONING VECTORS [J].

CHURCHWARD, G ;

BELIN, D ;

NAGAMINE, Y .

GENE, 1984, 31 (1-3) :165-171

[4] GENETIC-BASIS OF MINICELL FORMATION IN ESCHERICHIA-COLI K-12 [J].

DAVIE, E ;

SYDNOR, K ;

ROTHFIELD, LI .

JOURNAL OF BACTERIOLOGY, 1984, 158 (03) :1202-1203

[5]

DAYHOFF MO, 1983, METHOD ENZYMOL, V91, P524

[6] A DIVISION INHIBITOR AND A TOPOLOGICAL SPECIFICITY FACTOR CODED FOR BY THE MINICELL LOCUS DETERMINE PROPER PLACEMENT OF THE DIVISION SEPTUM IN ESCHERICHIA-COLI [J].

DEBOER, PAJ ;

CROSSLEY, RE ;

ROTHFIELD, LI .

CELL, 1989, 56 (04) :641-649

[7] CENTRAL ROLE FOR THE ESCHERICHIA-COLI MINC GENE-PRODUCT IN 2 DIFFERENT CELL DIVISION-INHIBITION SYSTEMS [J].

DEBOER, PAJ ;

CROSSLEY, RE ;

ROTHFIELD, LI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :1129-1133

[8] ISOLATION AND PROPERTIES OF MINB, A COMPLEX GENETIC-LOCUS INVOLVED IN CORRECT PLACEMENT OF THE DIVISION SITE IN ESCHERICHIA-COLI [J].

DEBOER, PAJ ;

CROSSLEY, RE ;

ROTHFIELD, LI .

JOURNAL OF BACTERIOLOGY, 1988, 170 (05) :2106-2112

[9]

Donachie W.D., 1987, ESCHERICHIA COLI SAL, P1578

[10] NEW METHOD FOR GENERATING DELETIONS AND GENE REPLACEMENTS IN ESCHERICHIA-COLI [J].

HAMILTON, CM ;

ALDEA, M ;

WASHBURN, BK ;

BABITZKE, P ;

KUSHNER, SR .

JOURNAL OF BACTERIOLOGY, 1989, 171 (09) :4617-4622

← 1 2 →