A SIMPLIFIED METHOD FOR SCREENING THE APOLIPOPROTEIN-E POLYMORPHISM

被引:11
作者
EICHNER, JE [1 ]
KULLER, LH [1 ]
FERRELL, RE [1 ]
KAMBOH, MI [1 ]
机构
[1] UNIV PITTSBURGH,GRAD SCH PUBL HLTH,DEPT HUMAN GENET,PITTSBURGH,PA 15261
关键词
APOLIPOPROTEIN-E; POLYMORPHISMS; APO-E METHODS; APO-E SCREENING;
D O I
10.1159/000153976
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A simple method for screening populations for the apolipoprotein E polymorphism which involves isoelectric focusing of delipidated samples on polyacrylamide gels of pH 4.5-5.8 followed by immunoblotting using a double-antibody technique is presented. The method is a synthesis of two previously published procedures and works well on samples that have been stored for as long as 15 years.
引用
收藏
页码:61 / 64
页数:4
相关论文
共 12 条
[1]   THE RECEPTOR MODEL FOR TRANSPORT OF CHOLESTEROL IN PLASMA [J].
BROWN, MS ;
GOLDSTEIN, JL .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1985, 454 :178-182
[2]   APOLIPOPROTEIN-E POLYMORPHISM AND ATHEROSCLEROSIS [J].
DAVIGNON, J ;
GREGG, RE ;
SING, CF .
ARTERIOSCLEROSIS, 1988, 8 (01) :1-21
[3]   PHENOTYPIC EFFECTS OF APOLIPOPROTEIN STRUCTURAL VARIATION ON LIPID PROFILES .3. CONTRIBUTION OF APOLIPOPROTEIN-E PHENOTYPE TO PREDICTION OF TOTAL CHOLESTEROL, APOLIPOPROTEIN-B, AND LOW-DENSITY-LIPOPROTEIN CHOLESTEROL IN THE HEALTHY WOMEN STUDY [J].
EICHNER, JE ;
KULLER, LH ;
FERRELL, RE ;
MEILAHN, EN ;
KAMBOH, MI .
ARTERIOSCLEROSIS, 1990, 10 (03) :379-385
[4]   PHENOTYPIC EFFECTS OF APOLIPOPROTEIN STRUCTURAL VARIATION ON LIPID PROFILES .4. APOLIPOPROTEIN POLYMORPHISMS IN A SMALL-GROUP OF BLACK-WOMEN FROM THE HEALTHY WOMEN STUDY [J].
EICHNER, JE ;
KULLER, LH ;
FERRELL, RE ;
KAMBOH, MI .
GENETIC EPIDEMIOLOGY, 1989, 6 (06) :681-689
[5]   ABNORMAL INVIVO METABOLISM OF APOLIPOPROTEIN-E4 IN HUMANS [J].
GREGG, RE ;
ZECH, LA ;
SCHAEFER, EJ ;
STARK, D ;
WILSON, D ;
BREWER, HB .
JOURNAL OF CLINICAL INVESTIGATION, 1986, 78 (03) :815-821
[6]  
GREGG RE, 1984, J LIPID RES, V25, P1167
[7]  
HAVEKES LM, 1987, J LIPID RES, V28, P455
[8]  
KAMBOH MI, 1988, J LIPID RES, V29, P1535
[9]   LIPOPROTEIN RECEPTORS AND CHOLESTEROL HOMEOSTASIS [J].
MAHLEY, RW ;
INNERARITY, TL .
BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 737 (02) :197-222
[10]   EDUCATIONAL-ATTAINMENT AND BEHAVIORAL AND BIOLOGIC RISK-FACTORS FOR CORONARY HEART-DISEASE IN MIDDLE-AGED WOMEN [J].
MATTHEWS, KA ;
KELSEY, SF ;
MEILAHN, EN ;
KULLER, LH ;
WING, RR .
AMERICAN JOURNAL OF EPIDEMIOLOGY, 1989, 129 (06) :1132-1144