INVOLVEMENT OF RH BLOOD-GROUP POLYPEPTIDES IN THE MAINTENANCE OF AMINOPHOSPHOLIPID ASYMMETRY

被引：67

作者：

SCHROIT, AJ ^{[1
]}

BLOY, C ^{[1
]}

CONNOR, J ^{[1
]}

CARTRON, JP ^{[1
]}

机构：

[1] INST NATL TRANSFUSION SANGUINE,INSERM,U76,F-75015 PARIS,FRANCE

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 45期

关键词：

D O I：

10.1021/bi00497a003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, 125I-azido-PS, and with an inhibitor of PS transport, 125I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS. © 1990, American Chemical Society. All rights reserved.

引用

页码：10303 / 10306

页数：4

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