AGE-RELATED CHANGES IN FTIR AND RAMAN SPECTRA OF HUMAN BLOOD

被引：14

作者：

Makhnii, T. ^{[1
]}

Ilchenko, O. ^{[1
]}

Reynt, A. ^{[1
]}

Pilgun, Y. ^{[2
]}

Kutsyk, A. ^{[2
]}

Krasnenkov, D. ^{[1
]}

Ivasyuk, M. ^{[3
]}

Kukharskyy, V. ^{[1
]}

机构：

[1] NAMS Ukraine, DF Chebotarev State Inst Gerontol, 67 Vyshhorodska Str, UA-04114 Kiev, Ukraine

[2] Taras Shevchenko Natl Univ Kyiv, Fac Radio Phys Elect & Comp Syst, 2 Prosp Acad Glushkov, UA-03022 Kiev, Ukraine

[3] Natl Univ Kyiv, Mohyla Acad, Fac Life Sci, 2 G Skovoroda Str, UA-04655 Kiev, Ukraine

来源：

UKRAINIAN JOURNAL OF PHYSICS | 2016年 / 61卷 / 10期

关键词：

human blood; age; Raman spectroscopy; ATR-FTIR spectroscopy; Partial Least Squares (PLS) analysis;

D O I：

10.15407/ujpe61.10.0853

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Blood analysis by spectroscopic techniques can provide important information about biochemistry and life processes in it. Blood indices are highly variable, and plenty of factors influence them. The present work describes the combination of two methods - IR and Raman spectroscopies of blood applied to investigate gerontology issues. We carried out a pilot study of 74 blood samples. The donors were differentiated by age with the Partial Least Squares (PLS) analysis of Raman and IR spectra. Analyzing the principal component spectra obtained during PLS processn the most illustrative bands were found in the intervals 2860-3030 cm(-1), 1370-1620 cm(-1), 1020-1220 cm(-1) and in 1650-1530 cm(-1), 1380-1360 cm(-1), 1220-1200 cm(-1), 1002-1004 cm(-1), 760-750 cm(-1) in IR and Raman spectral regions, respectively. Calibration models obtained via the PLS analysis of blood vibrational spectra provide the accuracy of age determination around +/-15 years from FTIR data and around +/-20 years from Raman data. Though such calibrations cannot be used for the precise determination of age, the age-related changes of blood do really exist and can be detected from vibrational spectra.

引用

页码：853 / 862

页数：10

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