DIRECT AUTOMATED SEQUENCING OF 16S RDNA AMPLIFIED BY POLYMERASE CHAIN-REACTION FROM BACTERIAL CULTURES WITHOUT DNA PURIFICATION

被引：348

作者：

HIRAISHI, A

机构：

[1] Laboratory of Environmental Biotechnology, Konishi Co, Ltd, Tokyo, 130, 5-6-3 Yokokawa, Sumida-ku

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1992年 / 15卷 / 05期

关键词：

D O I：

10.1111/j.1472-765X.1992.tb00765.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 16S rRNA gene from various bacterial cultures was amplified by the polymerase chain reaction without DNA purification, and sequenced directly by using a laser fluorescent DNA sequencer and Tth polymerase with a cycle sequencing protocol. The described procedures provide almost complete 16S rDNA sequence data within a couple of days and facilitate systematic studies.

引用

页码：210 / 213

页数：4

共 9 条

[1] RAPID-DETERMINATION OF BACTERIAL RIBOSOMAL-RNA SEQUENCES BY DIRECT SEQUENCING OF ENZYMATICALLY AMPLIFIED DNA [J].