EMISSION-SPECTRUM OF THE MICROSOMAL CHEMI-LUMINESCENCE OF A PROXIMATE CARCINOGEN, 7,8-DIOL-BENZO[A]PYRENE, DETERMINED WITH A WEDGE INTERFERENCE FILTER SPECTROMETER

被引：13
作者：
HAMMAN, JP
BIGGLEY, WH
SELIGER, HH
机构：
[1] JOHNS HOPKINS UNIV,MCCOLLUM PRATT INST,BALTIMORE,MD 21218
[2] JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218
来源：
PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1979年 / 30卷 / 04期
关键词：
D O I：
10.1111/j.1751-1097.1979.tb07172.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Abstract— The emission spectrum of the microsome‐mediated chemiluminescence of a proximate carcinogenic metabolite of benzo[a]pyrene, 7,8‐diol‐benzo[a]pyrene. has been measured with a wedge interference filter spectrometer calibrated with known chemiluminescent and bioluminescent reactions. This instrument has a noise equivalent signal at 490 nm (bandpass 24 nm) of 104 photon s‐1 at 20°C. Spectral and kinetic data indicate that the microsomal chemilurninescence of this metabolite is an exiplex chemiluminescence that proceeds through a dioxetane intermediate. Copyright © 1979, Wiley Blackwell. All rights reserved
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页码：519 / 524
页数：6
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