EASY ASSEMBLY OF LIGANDS FOR GLYCOSIDASE AFFINITY-CHROMATOGRAPHY

被引：9

作者：

BERNOTAS, RC ^{[1
]}

GANEM, B ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT CHEM,BAKER LAB,ITHACA,NY 14853

来源：

BIOCHEMICAL JOURNAL | 1990年 / 270卷 / 02期

关键词：

D O I：

10.1042/bj2700539

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved, high-yield synthesis of the corresponding N-carboxypentyl derivatives of three iminoalditol glycosidase inhibitors has been developed for affinity chromatography enzyme purification. Reductive amination of 1-deoxynojirimycin (or its D-manno or D-galacto analogues) with methyl 5-formylvalerate and NaBH3CN at neutral pH afforted an aminoester which upon hydrolysis with aqueous 5% HCl gave the desired amino acid in 97% overall yield. These amino acids could then be covalently attached using water-soluble carbodi-imide to 6-aminohexyl Sepharose 4B.

引用

页码：539 / 540

页数：2

共 7 条

[1]

FELLOWS L, 1989, NEW SCI, V123, P45

[2] PURIFICATION BY AFFINITY-CHROMATOGRAPHY OF GLUCOSIDASE-I, AN ENDOPLASMIC-RETICULUM HYDROLASE INVOLVED IN THE PROCESSING OF ASPARAGINE-LINKED OLIGOSACCHARIDES [J].