UV INDUCED (6-4) PHOTOPRODUCTS ARE DISTRIBUTED DIFFERENTLY THAN CYCLOBUTANE DIMERS IN NUCLEOSOMES

被引：82

作者：

GALE, JM ^{[1
]}

SMERDON, MJ ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,BIOCHEM BIOPHYS PROGRAM,PULLMAN,WA 99164

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1990年 / 51卷 / 04期

关键词：

D O I：

10.1111/j.1751-1097.1990.tb01732.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— We have compared the distributions of two stable UV photoproducts in nucleosome core DNA at the single‐nucleotide level using a T4 polymerase‐exonuclease mapping procedure. The distribution of pyrimidine‐pyrimidone (6‐4) dimers was uncovered by reversing the major UV photo‐product, cis‐syn cyclobutane pyrimidine dimer, with E. coli DNA photolyase and photoreactivating light. Whereas the distribution of total UV photoproducts in nucleosome core DNA forms a striking 10.3 base periodic pattern, the distribution of (6‐4) dimers is much more random throughout the nucleosome core domain. Therefore, histone‐DNA interactions in nucleosomes strongly modulate formation of the major class of UV‐induced photoproducts, while having either a constant effect or no effect on (6‐4) dimer formation. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：411 / 417

页数：7

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