THE EXTREME CARBOXYL-TERMINUS OF THE EQUINE HERPESVIRUS-1 HOMOLOG OF HERPES-SIMPLEX VIRUS VP16 IS ESSENTIAL FOR IMMEDIATE-EARLY GENE ACTIVATION

被引:21
|
作者
ELLIOTT, GD [1 ]
机构
[1] UNIV LEEDS,DEPT MICROBIOL,MOLEC VIROL GRP,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1128/JVI.68.8.4890-4897.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Gene 12 of equine herpesvirus 1 (EHV-1), the homolog of herpes simplex virus (HSV) VP16 (alpha TIF, Vmw65), was cloned into a eukaryotic expression vector by PCR and used in transactivation studies of both the EHV-1 and HSV-1 IE1 promoters. Results demonstrated that the product of gene 12 is a potent transactivator of immediate-early gene expression of both viruses, which requires sequences in the upstream HSV-1 promoter for activity. Mutational analysis of the gene 12 open reading frame indicated that removal of the C-terminal 7 amino acids, which contain a short region of homology with the extreme C terminus of VP16, inactivated the protein. Within this region, only a single methionine residue appeared to be essential for activity, implying that gene 12 may have a modular array of organization similar to that of VP16. However, fusion of the gene 12 C terminus to a truncated form of VP16, which contained the complex formation domain, did not restore activity to the HSV-1 protein. These data demonstrate that the EHV-1 immediate-early transactivator may not be functionally colinear with VP16, with transactivation requiring both the C terminus and another region(s) present within the N-terminal portion.
引用
收藏
页码:4890 / 4897
页数:8
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