PROPERTIES OF OMEGA-CONOTOXIN MVIIC RECEPTORS ASSOCIATED WITH ALPHA-1A CALCIUM-CHANNEL SUBUNITS IN RAT-BRAIN

被引：32

作者：

MARTINMOUTOT, N ^{[1
]}

LEVEQUE, C ^{[1
]}

SATO, K ^{[1
]}

KATO, R ^{[1
]}

TAKAHASHI, M ^{[1
]}

SEAGAR, M ^{[1
]}

机构：

[1] MITSUBISHI KASEI INST LIFE SCI,MACHIDA,TOKYO 194,JAPAN

来源：

FEBS LETTERS | 1995年 / 366卷 / 01期

关键词：

CALCIUM CHANNEL; OMEGA CONOTOXIN MVIIC RECEPTOR; ALPHA-1A SUBUNIT; ALPHA-2; SUBUNIT;

D O I：

10.1016/0014-5793(95)00467-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Solubilized I-125-omega conotoxin MVIIC receptors from rat cerebellum were immunoprecipitated by antibodies directed against the calcium channel alpha 1A subunit. Anti-alpha 1A antibodies recognized a 240-220, 180 and 160 kDa proteins in immunoblots of cerebellar membranes. Disuccinimidyl suberate cross-linked I-125-omega conotoxin MVIIC to an alpha 2 delta-like 200-180 kDa subunit, which migrated at 150-140 kDa after disulfide reduction. These observations are consistent with a heteromeric structure in which high affinity omega conotoxin MVIIC binding sites formed by alpha 1A subunits are located in close proximity to peripheral alpha 2 subunits.

引用

页码：21 / 25

页数：5

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