STRUCTURAL CHARACTERIZATION OF CANINE PYY

被引：38

作者：

EYSSELEIN, VE

EBERLEIN, GA

GRANDT, D

SCHAEFFER, M

ZEHRES, B

BEHN, U

SCHAEFER, D

GOEBELL, H

DAVIS, M

LEE, TD

SHIVELY, JE

MEYER, HE

REEVE, JR

机构：

[1] CALIF BIOTECHNOL INC,MT VIEW,CA 94043

[2] UNIV ESSEN GESAMTHSCH,DEPT GASTROENTEROL & SURG,W-4300 ESSEN 1,GERMANY

[3] CITY HOPE NATL MED CTR,BECKMAN RES INT,DIV IMMUNOL,DUARTE,CA 91010

[4] RUHR UNIV BOCHUM,INST PHYSIOL CHEM 1,W-4630 BOCHUM,GERMANY

[5] UNIV CALIF LOS ANGELES,VET ADM WADSWORTH MED CTR,CTR ULCER RES & EDUC,DEPT MED,LOS ANGELES,CA 90073

来源：

PEPTIDES | 1990年 / 11卷 / 01期

关键词：

Fast protein liquid chromatography; Gastric acid secretion; gastrointestinal hormones; High performance liquid chromatography; Microsequence analysis; Molecular heterogeneity; Motility; Neuropeptide Y (NPY); Pancreatic secretion; Peptide YY (PYY); Radioimmunoassay;

D O I：

10.1016/0196-9781(90)90118-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PYY was purified from canine colonic mucosa by sequential steps of reverse phase HPLC and ion-exchange FPLC. Microsequence, amino acid and mass spectral analyses of the purified peptide and its tryptic fragments were consistent with the structure: YPAKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY-amide. Canine PYY(1-36) has the identical sequence as porcine and rat PYY but differs from human PYY at position 3, with Ala instead of Ile, and position 18, with Ser instead of Asn. A smaller form, PYY(3-36), was also purified and characterized. It may differ in its biological activity from the intact peptide and could act as a partial antagonist or agonist of PYY(1-36). © 1990.

引用

页码：111 / 116

页数：6

共 30 条

[11]

HAWKE D, 1982, ANAL BIOCHEM, V120, P302, DOI 10.1016/0003-2697(82)90351-7

[12] CHARACTERIZATION OF PEPTIDE-YY RECEPTORS IN THE BRAIN [J].