SYNTHESIS AND REACTIONS OF HYDROPHILIC FUNCTIONAL MICROSPHERES FOR IMMUNOLOGICAL STUDIES

There is a need for hydrophilic polymeric microspheres with functional groups on their surface which can be reacted efficiently with proteins. These microspheres with antibodies (immunoglobulins) covalently bound to their surfaces constitute valuable immunoreagents capable of marking specific receptors (antigens) on cell surface membranes. The main requirements of the microspheres for the above applications are: insolubility in aqueous or organic media, absence of aggregation and of nonspecific interaction with cells and presence of suitable functional groups for covalent. © 1979, Taylor & Francis Group, LLC. All rights reserved.