THE BUDDING YEAST HRR25 GENE-PRODUCT IS A CASEIN KINASE-I ISOFORM

被引：78

作者：

DEMAGGIO, AJ ^{[1
]}

LINDBERG, RA ^{[1
]}

HUNTER, T ^{[1
]}

HOEKSTRA, MF ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO, DEPT BIOL, LA JOLLA, CA 92093 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 15期

关键词：

SACCHAROMYCES-CEREVISIAE; PROTEIN PHOSPHORYLATION;

D O I：

10.1073/pnas.89.15.7008

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Saccharomyces cerevisiae HRR25 gene was identified as a regulator of DNA strand-break repair. HRR25 encodes a protein kinase that is closely related to bovine casein kinase I (CKI). CKI is a ubiquitous multipotential protein kinase. Rabbit polyclonal antibodies that recognize and immunoprecipitate Hrr25p have been generated and an immune complex protein kinase assay has been developed. The reaction depends upon HRR25 and shows that Hrr25p uses casein as a substrate. The identity between Hrr25p and bovine CKI suggests that Hrr25p is a yeast isoform of the CKI family and that CKIs may play a role in regulating DNA metabolism.

引用

页码：7008 / 7012

页数：5

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